Enzyme Kinetics

Competitive Inhibition - Ki


For competitive inhibition there is one more important constant, the Ki, or inhibition constant, which is the dissociation constant for the EI complex.

Ki = ([E][I])/[EI]

If 1/v is plotted against inhibitor concentration [I], a staight line plot is obtained for each substrate concentration [S]. If the the line for several different [S] converge in the left upper quadrant the inhibitor is competitive. If the converge on the [I] axis, the inhibitor is non-competitive. These plots are called Dixon plots.

The inhibitor constant Ki is the concentration of inhibitor which is required to decrease the maximal rate of the reaction to half of the uninhibited value, in the presence of a low substrate concentration.

Therefore, the lower the Ki the lower the concentration of inhibitor needed to lower the rate.


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